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. 2021 Jan 7;10(2):e12038. doi: 10.1002/jev2.12038

FIGURE 3.

FIGURE 3

Western blotting of control and macroalbuminuria urinary extracellular vesicle samples isolated by Hydrostatic Filtration Dialysis (HD), ultracentrifugation (UC) and Norgen Kit (NG). EVs from 2 ml of urine were loaded from each method. Immunodetection of EV markers (TSG101, CD9 and podocalyxin, PDX) and two common proteins that co‐isolate with urinary extracellular vesicles (Tamm–Horsfall protein, THP and albumin, ALB) are shown