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. 2021 Jan 6;22:22. doi: 10.1186/s12864-020-07345-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Representative GUS staining patterns to define a promoter region directing anther-specific expression of BoGH3.13–1. a Genomic DNA regions used in transgenic lines for promoter analysis. Names of transgenic plants used are written in italic and different regions upstream of BoGH3.13–1 start codon to direct GUS reporter expression are indicated in parentheses. b-f GUS staining of floral buds (b), a dissected floral bud (c), open flowers (d), siliques (e), and 8-day old seedling (f) of P1 transgenic plants, and floral buds from P2 (g), P3 (h), P4 (i), and P5 (j) are shown. Arrows indicate GUS-stained pollen (d) or floral abscission zones in a silique (e). Dividends and denominators of fractions in the pictures are numbers of transgenic plants with the GUS staining and all the transgenic plants examined, respectively. Scale bars: 1 mm