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Thermal cycle conditions for KASP genotyping reactions
| Step | Description | Temperature | Time | Cycles |
|---|---|---|---|---|
| 1 | Activation | 94 °C | 15 min | 1 cycle |
| 2 | Denaturation | 94 °C | 20 s | 10 cycles |
| Annealing/elongation | 61–55 °C (drop 0.6 °C per cycle) | 60 s | ||
| 3 | Denaturation | 94 °C | 20 s | 35 cycles |
| Annealing/elongation | 55 °C | 60 s | ||
| 4 | Read stage (FAM™, HEX™, ROX™) | 30 °C | 60 s |
