Fig. 2.

Inheritance patterns of CRISPR/Cas9 induced gene deletion of GALT3 in the T1, T2, and T3 generations in selected transgenic lines. a. Locations of the two primers (F/R) used to amplify the gene fragment covering the two target sites (3–1 and 3–2) of GALT3. b. Three T1 CRISPR lines (#12, #25, and #40) containing partial deletions in GALT3. c. Segregation patterns of gene editing in two T2 transgenic lines (#25 and #40) showing partial and complete (red numbers) gene deletions of GALT3. d. Stable transmission of gene editing of the #40–10 transgenic line in the T3 generation showing complete deletions in all progeny. M corresponds to the DNA ladder, red numbers indicate transgenic lines that contained a full deletion in the GALT3 gene