Fig. 3.

Efficient gene editing of all given target sites of galt2 galt3 galt4 galt5 galt6 mutant line #40–10. (From top to bottom) Two gRNAs (3–1 and 3–2) created a 581 bp deletion in GALT3; GALT4 contained a 1 bp insertion in the last exon; GALT6 contained 1 bp insertions at both 6–1 and 6–2 target sites. All mutations resulted in frame-shifts and pre-mature stop codons. Mutation sites are indicated by red arrows on the sequencing chromatograms on the right