Fig. 4.

Multiplex gene-editing events occurred in three gRNAs of galt2 galt3 galt4 galt5 galt6 mutant line #29–36. (From top to bottom) The 3–3 gRNA generated a 97 bp deletion in GALT3; the 4–2 and 4–3 gRNAs created a 1 bp deletion and a 1 bp insertion in the GALT4 gene, respectively; the 6–3 gRNA produced a 4 bp indel mutation in the GALT6 gene. All mutations resulted in frame-shifts (red amino acids) and pre-mature stop codons (asterisks) in their respective protein sequences. Mutation sites are indicated by red arrows on the sequencing chromatograms on the right