Table 2.
All antibodies used with information on species, isotype, class, catalogue number and working dilution. Figure shows antibody combinations used for (a) α-sarcoglycan (b) β-dystroglycan and (c) f/d myosin triple stains
| Primary antibody | Species | Class | Catalogue # | Dilutions |
|---|---|---|---|---|
| β-dystroglycan | Mouse IgG2a | Monoclonal | NCL-b-DG | 1:20 |
| Dystrophin (exon 77) | Rabbit IgG | Polyclonal | ab15277 | 1:200 |
| Laminin α2-1 (300 kDa) | Rat IgG1 | Monoclonal | 804-190-C100 | 1:50 |
| Myosin developmental | Mouse IgG1 | Monoclonal | NCL-MHCd | 1:30 |
| Myosin neonatal | Mouse IgG1 | Monoclonal | NCL-MHCn | 1:30 |
| α-sarcoglycan | Mouse IgG1 | Monoclonal | NCL-a-SARC | 1:40 |
| Secondary antibody | Species | Class | Catalogue # | Dilutions |
| Alexa Fluor 488 | Goat anti-Rabbit IgG | Polyclonal | A-11034 | 1:100 |
| Alexa Fluor 568 | Goat anti-Rat IgG | Polyclonal | A-11077 | 1:100 |
| Alexa Fluor 647 | Donkey anti-Mouse IgG | Polyclonal | A-31571 | 1:100 |
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