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. 2021 Jan 6;19:9. doi: 10.1186/s12951-020-00749-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — In vitro anti-tumor therapy of IGM. a Representative fluorescence images of hypoxia (red) in 4T1 tumor cells after various treatments. b Fluorescence images and Flow cytometry quantitative analysis of ROS generation stained with DCFH-DA (green) and the nucleus stained with DAPI (blue) after different treatments. Scale bar is 100 μm. c Tumor cell viability after various treatments d Western blot of HSP90 expression in different treatments. Data are expressed as mean ± SD (n = 3)