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. 2021 Jan 6;9:9. doi: 10.1186/s40478-020-01111-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — ALS-linked mutant FUS transgene is restricted to neurons and has cytosolic localization. a Schematic of FUS transgene. Murine prion promoter was used to drive the HA-tagged human FUS cDNA expression (R514G). b Schematic representation of different subregions (CA1, CA3 and DG) within hippocampus, and used for panels c–h. Confocal images of endogenous FUS (magenta) and human FUS transgene (red) in the CA1 (c), CA3 (d) and DG (e) of the hippocampus at 3 months of age. Transgenic FUS protein was detected using a HA antibody. Both endogenous FUS and human FUS transgene were present in the neuronal nucleus. Scale bar is 20 μm. Confocal images of the CA1 (f), CA3 (g), and DG (h) regions that were co-labeled with endogenous FUS (magenta) or R514G-FUS transgene (red) and nuclear envelope marker (green) from non-transgenic and R514G mice at 3 months of age. Endogenous FUS (magenta) is restricted to nuclei, whereas R514G-FUS transgene (red) showed both nuclear and cytosolic distribution. The nuclear envelope appeared to be normal across all regions of the hippocampus. Scale bar = 20 μm