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. 2021 Jan 6;9(1):e001402. doi: 10.1136/jitc-2020-001402

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Improved immune response by CompK in dose finding study with tumor-bearing mice. (A) Inhibition of SLP76 phosphorylation by CompK. Compound was administrated to mice bearing 1956 sarcoma at 30 mpk and 100 mpk doses, two times per day for 5 days. Blood was harvested at the end of the study followed by analysis of pSLP76 in T cells by flow cytometry. (B–D) Enhanced levels of IFN-γ and T-cell activation markers by CompK treatment. Blood samples were used to prepare messenger RNA and complementary DNA. Quantitative PCR analysis was conducted to evaluate IFN-γ, CD25, and CD69 expression after treatment with vehicle or CompK. (E) IC50 of CompK on SLP76 phosphorylation in mouse whole blood. (F) Analysis of blood levels of CompK over time. Blood samples were collected at 1, 3, 7 and 24 hours post compound dosing. CompK levels were measured by liquid chromatography with tandem mass spectrometry. CompK, compound K; IFN-γ, interferon gamma; ns, not significant; WB, whole blood.