Figure 7.

Mechanistic translation from mouse to human tumors. (A) Improved proinflammatory gene signatures by CompK and anti-PD1 in CT26 tumor explant study. Randomized CT26 tumor explants were treated by 0.5 µM CompK, 1 µg/mL anti-PD1 or a combination of CompK plus anti-PD1 for 72 hours. Explants were harvested followed by qPCR analysis. Each data point was generated from eight explants. Statistical significance was generated by comparing groups of anti-PD1 alone and anti-PD1 plus CompK. (B) Enhanced levels of IFN-γ and GZMB by CompK in human CRC explant study. Tumor explants were prepared with fresh human CRC tumors. The randomized tumor explants were treated by 0.5 µM CompK for 72 hours followed by qPCR analysis. Data were generated with tumor samples from seven patients. Student t-test was used for statistical analysis. *P≤0.05, **P<0.01. Data are presented as mean±SEM. CompK, compound K; CRC, colorectal carcinoma; DMSO, dimethyl sulfoxide; IFN-γ, interferon gamma; IL, interleukin; ns, not significant; qPCR, quantitative PCR.