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Immunofluorescence identification of SCNs. (A) Cell nuclei are identified with DAPI. (B) SCNs are stained with the anti-NF200 monoclonal antibody. More than 90% of the cultured cells show positive expression of NF200 on the day 7 of ex vivo culture. The number of NF200-positive neurons are counted per area and expressed as percentage of total number of cells (Scale bar: 40 μm).
