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Table 3.
PCR cycling programme
| Step | Temp °C | Time |
|---|---|---|
| 1 | 94 | 3 min |
| 2 | 94 | 30 s |
| 3 | 58 | 45 s |
| 4; go to step 2, 35 cycles | 72 | 30 s |
| 5 | 72 | 2 min |
| 6 | 4 | Hold |