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. 2021 Jan 7;18:1. doi: 10.1186/s12950-020-00266-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — The attenuation of lncH19 by miR-324-3p was inhibited as the inflammatory response attenuated under treatment with IL-1β (10 ng/mL) and Dex (1 μM) at the same time. a. Levels of the candidate miRNAs, as determined via RT-PCR, for examining the performance of the miRNA inhibitor. b. Cell proliferation was determined using the MTS assay. c. Cell apoptosis was determined via flow cytometry. d. mRNA levels of the inflammatory cytokines. e. ELISA for detecting the levels of the inflammatory cytokines. f. Western blot for detecting the levels of the inflammatory genes. Data are presented as mean ± SD. N = 3. *P < 0.05, **P < 0.01. Dex: dexamethasone. IL-1β: interleukin-1 β; NC + miRNA NC: shNC + IL-1β + Dex + miRNA NC; NC + miR-324-3p inhibitor: shNC + IL-1β + Dex + miR-324-3p inhibitor; shlncH19 + miRNA NC: shlncH19 + IL-1β + Dex + miRNA NC; shlncH19 + miR-324-3p inhibitor: shlncH19 + IL-1β + Dex + miR-324-3p inhibitor