Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 6;40:16. doi: 10.1186/s13046-020-01810-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — Exosomes regulate the proliferation and invasion of glioma through miR-375-CTGF-EGFR signalling pathway. a. Changes in miR-375 levels in miR-375 overexpressing U87 and U251 cells treated with or without GW4869 were examined by qRT-PCR. b-c. CCK-8 and EdU analysis of the effect elicited by GW4869 on the inhibitory effect of miR-375 on the proliferation of U87 and U251 cells. Scale bar = 100 μm. d. Wound healing analysis demonstrating the effect of GW4869 on the inhibitory effect of miR-375 on the migration of U87 and U251 cells. Scale bar = 400 μm. e. Transwell analysis showing the effect of GW4869 on the inhibitory effect of miR-375 on glioma cell migration (left) and invasion (right). Scale bar = 100 μm. f. Western blot analysis of CTGF, p-EGFR (Tyr1068), EGFR, p-AKT (Ser-473), AKT, and MMP9 in U87 and U251 cells. g. Effect of monensin on the inhibitory effect of miR-375 on CTGF and its downstream molecules p-EGFR (Tyr1068), p-AKT (Ser-473), and MMP9. All experiments were repeated independently three times. Data are presented as mean ± standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001. ns, not significant