Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 6;19:12. doi: 10.1186/s12967-020-02572-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — PGM5-AS1 overexpression inhibits the proliferation and colony formation of PC-3 and DU-145 cells and promotes their apoptosis. a The relative expression of PGM5-AS1 in PC-3 and DU-145 cells after transfection through RT-qPCR assay. b The representative images of cell proliferation in transfected PC-3 cells as measured by EdU assay (×200). c The quantitative analysis of cell proliferation in transfected PC-3 cells as measured by EdU assay. d The protein bands of expression of Ki67 and PCNA in transfected PC-3 cells through Western blot analysis. e The quantitative analysis of expression of Ki67 and PCNA in transfected PC-3 cells through Western blot analysis. f The representative images of cell colony formation in transfected PC-3 cells. g The quantitative analysis of cell colony formation in transfected PC-3 cells. h The flow cytometric detection of cell apoptosis in transfected PC-3 cells. i The quantitative analysis of cell apoptosis in transfected PC-3 cells detected by flow cytometric analysis. j The quantitative analysis of expression of cleaved caspase-3, Bax, RIP3, cyclophilinA and Bcl-2 in transfected PC-3 cells through Western blot analysis. k LDH release determined by ELISA. *p < 0.05 vs. the oe-NC group (PCa cells treated with oe-NC). The measurement data were summarized as mean ± standard error and analyzed using one-way ANOVA, followed by Tukey's post hoc test. The experiment was repeated three times independently