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. 2021 Jan 6;19:12. doi: 10.1186/s12967-020-02572-w

Table 1.

Primer sequences for RT-qPCR

Gene Forward sequence Reverse sequence
PGM5-AS1 5′-GACTATGTTGTGAGCCTGCG-3′ 5′-AAAAGGGGAGGGGCAATACA-3′
miR-587 5′-CCAGGCAAGAGAGAGTTGCTG-3′ 5′-AGTCACAGGTGCAGACACATT-3′
GDF10 5′-GGACTTTGACGAGAAGACGATG-3′ 5′-TCTTAGGCATGGGGAACTCAC-3′
U6 5′-CTCGCTTCGGCAGCACA-3′ 5′-AACGCTTCACGAATTTGCGT-3′
β-actin 5′-CCTGGCACCCAGCACAAT-3′ 5′-GCCGATCCACACGGAGTACT-3′

RT-qPCR reverse transcription quantitative polymerase chain reaction, PGM5-AS1 homo sapiens PGM5 antisense RNA 1, miR microRNA, GDF10 growth and differentiation factor 10