Fig. 1.

CASC15 is highly expressed in NSCLC and is essential for tumor cell migration and growth. a Relative RNA levels of CASC15 in primary human NSCLC tissues and matched adjacent normal tissues. (GEO: GDS3837, n = 60 for each group). b Kaplan-Meier curves representing the probabilities of overall survival in a cohort of 293 lung cancer patients (GEO: GSE30219) stratified according to the expression levels of CASC15. c Relative RNA levels of CASC15 (mean ± SEM, n = 3) in five NSCLC cell lines (H1650, H1299, H1975, A549, H520) and one normal human bronchial epithelial cell line BEAS-2B. d Transwell migration assay of A549 and H1299 cells treated with si-Control or si-CASC15 for 72 h. e FITC-phalloidin staining of A549 and H1299 cells treated with si-Control or si-CASC15 for 72 h, followed by confocal microscopic imaging. Scale bar: 30 μm. f Cell proliferation curves of A549 and H1299 cells treated with si-Control or si-CASC15. The experiment was carried out in quintuplicate wells and repeated at least twice. g Bioluminescence imaging in human NSCLC xenograft model. A549 or H1299 cells with stable knockdown of CASC15 were injected subcutaneously into nude mice (n = 6 per group). Afterward, primary tumor growth was monitored by regular measurements using a digital caliper