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. 2021 Jan 6;21:14. doi: 10.1186/s12935-020-01698-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — PCAT6 was mainly located in the cytoplasm, and it regulated cell viability, migration, invasion, proliferation, and miR-139-3p expression in PA cells. a, b The transfection efficiencies of shPCAT6 in RC-4B/C and GH3 cells were determined by RT-qPCR. GAPDH was used as an internal control. c, d The expression location of PCAT6 in RC-4B/C and GH3 cells were determined by RT-qPCR. GAPDH and U6 were used as internal controls. e, f The expressions of miR-139-3p in RC-4B/C and GH3 cells after transfection were detected by RT-qPCR. U6 was used as an internal control. g, h The viability of RC-4B/C and GH3 cells after transfection was detected by CCK-8 assay. i, l–m The migration of RC-4B/C and GH3 cells after transfection was detected by wound healing assay (Magnification × 100). j, n, o The invasion of RC-4B/C and GH3 cells after transfection was detected by transwell assay (Magnification × 250). k, p, q The proliferation of RC-4B/C and GH3 cells after transfection was detected by colony formation assay. (^***P < 0.001, vs. shNC; ^###P < 0.001, vs. nuclear). shNC negative control of shRNA