Fig. 4.

Afh/Afh mice show specific methylation enzyme expression anomalies in the different nodes of the retinohypothalamic tract (RHT). a Retinal expression levels of the transcripts for genes involved in DNA methylation in the Afh/Afh (red) and +/+ (blue) genotypes; (minimum n = 7 for each genotype). b Expression levels of methylation enzymes in the SCNs of Afh mutants (red) and controls (blue) (minimum n = 18 for each genotype). c Hypothalamic expression levels of the transcripts of methylation enzymes in Afh mutants (red) and controls (blue) (n = 12 for each genotype). d RT-qPCR of methylation-mediating enzymes at the two different time points, ZT0 and ZT12; (minimum n = 7 per genotype at each time point). All expression levels are normalized to those of multiple housekeeping genes. All bars represent the average ± SEM for at least two different experiments. * p < 0.05, **p < 0.01, two-way ANOVA plus Bonferroni post-test (panel D), Student’s t test (panels A–C)