Fig. 5.

Rev-ERBα is involved in the transcriptional regulation of DNA methylation enzymes in vitro. a ChIP for Rev-ERBα in primary neuronal cells. Expression is normalized to the input. The bars represent the average values ± SEMs for two different experiments (n = 3 samples for each experiment pooled together for immunoprecipitation). b RT-qPCR of DNA methylation enzymes in primary neuronal cultures treated with a scramble siRNA and a specific siRNA against REV-ERBα. The bars represent the average values ± SEMs for three different experiments. c Drug treatment with the REV-ERBα agonist GSK 4112 at 10 μM of the drug on primary neurons of 8 DIVs (days in vitro). The bars represent the average values ± SEMs for two different experiments. The data were normalized on vehicle control (DMSO). * p < 0.05, ** p < 0.01, **** p < 0.0001, Student’s t test