Figure 1. Multiple-sequence alignment of the N-terminus of C3-, C3/C2-, and C2-like toxins.

(A) Residues of the S-motif, conserved in all groups, are shown in purple; residues conserved in C3- and C3/C2-like toxins are shown in pink; residues of the B-motif, conserved in C3/C2- and C2-like toxins, are shown in yellow; residues conserved in C3-like toxins are shown in gray. Bold residues denote deviations from otherwise conserved positions. Gaps within the sequence are denoted by (.) and continuation within a sequence is denoted by (-). Sequence numbers correspond to C3larvinA. (B) Graphical depiction of α₁-helix length in each N-terminal deletion variant. Sequence numbers correspond to C3larvinA.