Figure 5. C3larvinA N-terminal deletions have varying effects on the morphology of macrophage cells.

J774A.1 mouse macrophage cells were treated with 300 nM toxin and incubated for 20 h at a cell density of 37500 cells/well. Effects to morphology were less dramatic in the larger N-terminal deletions. (A) Buffer control; (B) C3larvinA (full length); (C) ΔY2-A21; (D) ΔY2-D23; (E) ΔY2-K25; (F) ΔY2-D27; (G) ΔY2-A30; (H) ΔY2-K33; (I) ΔY2-W34.