. 2021 Jan 6;41(1):BSR20203727. doi: 10.1042/BSR20203727

Table 1. Binding affinity and GH activity of C3LarvinA WT and N-terminal variants against NAD⁺.

C3larvinA	K_M (μM)	k_cat (min⁻¹) × 10⁻³	k_cat/K_M (M⁻¹.min⁻¹) × 10¹	K_D (μM)
WT	107 ± 20	261 ± 20	244	56 ± 11
ΔY2-A21	120 ± 31	100 ± 10	83	59 ± 2
ΔY2-D23	160 ± 23	46 ± 4	31	117 ± 10
ΔY2-K25	104 ± 27	19 ± 2	18	102 ± 3
ΔY2-D27	112 ± 17	12 ± 0.7	11	93 ± 5
ΔY2-A30	84 ± 17	7 ± 0.4	8	94 ± 3
ΔY2-K33	∼0	∼0	∼0	75 ± 7
ΔY2-W34	∼0	∼0	∼0	38 ± 9
F24A/A31L/W34A	∼0	∼0	∼0	85 ± 14
D23A/K25A/D27A/R28A	28 ± 6	46 ± 2	166	140 ± 14
D23A	94 ± 28	7 ± 0.5	7	131 ± 27
A31L	∼0	∼0	∼0	87 ± 11
K36E	72 ± 12	82 ± 5	114	38 ± 0.4
K36A	75 ± 18	58 ± 4	76	42 ± 2
G35T	∼0	∼0	∼0	113 ± 30
I153A	95 ± 13	40 ± 2	42	85 ± 8
Y178A	99 ± 12	8 ± 0.3	8	65 ± 14

Each value is the average of three replicates ± S.D.; ∼0 = not detectable.

Table 1. Binding affinity and GH activity of C3LarvinA WT and N-terminal variants against NAD+.