Fig. 2.

A: extracellular matrix protein expression in aorta of caveolin-1 knockout (KO) mice (Cav-1^−/−) and Cav-1 wild-type (WT) mice. Protein levels of extracellular matrix proteins (heparin sulfate, fibulin-5,collagen type 1) and actin in aortic tissue. Densitometric analysis is shown (n = 3). B: effect of in vitro copper (Cu) treatment on superoxide dismutase (SOD3)-specific activity purified from aortas of Cav-1-WT and Cav-1^−/− mice. Concanavalin A (Con A)-Sepharose-bound SOD3 or -unbound SOD1 proteins from aortas of Cav-1 WT and Cav-1^−/− mice were treated with or without CuCl₂ (10 mmol/L, 1 h at room temperature), and the specific activity of SOD3 and SOD1 was measured (n = 3) as described in Fig. 1. Results are presented as means ± SE. *P < 0.05 vs control. NS, not significant.