Fig. 1.
Efficacy of DREADD-mediated activation of ARC of AgRP and POMC neurons. A and B: schematics of the Cre-dependent hM3Dq-mCherry DREADD used (A) and stereotaxic targeted microinjection into hypothalamic ARC (B). C: representative images showing expression of mCherry and c-Fos in the ARC of AgRPCre (n = 13) and POMCCre (n = 9) mice treated intraperitoneally with CNO or vehicle. Scale bar, 100 µm. AgRP, agouti-related peptide; ARC, arcuate nucleus of the hypothalamus; POMC, proopiomelanocortin; CNO, clozapine-N-oxide; DREADD, designer receptor exclusively activated by designer drugs.