Fig. 1.

Experimental method. Cells were grown on permeable supports with three biological replicates as indicated. There were two technical replicates each, resulting in a total of 24 samples. Proteins were isolated, trypsinized, and labeled using isotopic tags (TMT-11 plex). The 24 samples were combined into 3 batches of 8 each with a shared sample pooled from all 24 samples. The combined samples were processed for proteomic and phosphoproteomic analysis as described in methods.