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Results of in vitro phosphorylation experiments. Protein extracts from PKA double knockout cells (PKA-Cα and PKA-Cβ) were incubated with recombinant PKA-Cα or PKA-Cβ, and phosphorylation was quantified by mass spectrometry. A: there was a marked similarity between responses to the two recombinant kinase proteins. B: sequence preference logos derived from the analysis were almost identical.
