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. 2020 Oct 5;319(5):F848–F862. doi: 10.1152/ajprenal.00383.2020

Table 3.

Phosphopeptides most convincingly changed in PKA-Cα-null cells versus PKA-Cα-intact [Pmod < 0.01 and |log(ratio)| > 0.5]

UniProt ID Gene Symbol† Amino Acid Number Annotation Sequence Log2 (PKA-Cα Null/ PKA-Cα Intact) Pmod Value Log2 (PKA-Cβ Null/ PKA-Cβ Intact) Pmod Value
P16254 Srp14 44 Signal recognition particle 14-kDa protein KS*SVEGLEPAENK −1.41 1.09 × 10−4 0.18 0.472
P31324 Prkar2b 112 cAMP-dependent protein kinase type II-β regulatory subunit RAS*VCAEAYNPDEEEDDAESR −1.38 0.001 −0.29 0.402
P05213 Tuba1b;
Tuba4a;
Tuba1a;
Tuba3a;
Tuba1c		 158 Tubulin α1B chain;
tubulin α4A chain;
tubulin α1A chain;
tubulin α1C chain		 LS*VDYGK −1.36 1.21 × 10−4 0.28 0.266
E9PVZ8 Golgb1 2655 Golgi autoantigen, golgin subfamily b, macrogolgin 1 KVS*EIEDQLK −1.29 0.001 0.20 0.484
Q68FG2 Sptbn2 2254 Spectrin β-chain RGS*LGFYK −1.28 0.003 0.20 0.576
P16254 Srp14 44 Signal recognition particle 14-kDa protein KS*SVEGLEPAENK −1.27 1.02 × 10−4 0.25 0.276
P14152 Mdh1 241 Malate dehydrogenase, cytoplasmic KLS*SAMSAAK −1.17 1.75 × 10−4 0.07 0.768
Q9ES28 Arhgef7 830 Rho guanine nucleotide exchange factor 7 S*LEEEQR −1.13 9.54 × 10−5 0.18 0.371
Q9DBC7 Prkar1a 83 cAMP-dependent protein kinase type I-α regulatory subunit EDEIS*PPPPNPVVK −1.05 1.34 × 10−4 −0.09 0.642
G3X9K3 Arfgef1 1076 Brefeldin A-inhibited guanine nucleotide-exchange protein 1 EGS*LTGTK −0.96 0.001 0.09 0.688
Q9CZX9 Emc4 36 Endoplasmic reticulum membrane protein complex subunit 4 SDRGS*GQGDSLYPVGYLDK −0.93 0.003 0.18 0.485
Q8CI52 Gramd1c 532 Protein aster-C SS*TDLGFEAK −0.92 3.38 × 10−4 −0.04 0.832
Q91XA2 Golm1 299 Golgi membrane protein 1 PEEDS*QYPER −0.91 0.008 0.08 0.794
Q9DBC7 Prkar1a 83 cAMP-dependent protein kinase type I-α regulatory subunit TDSREDEIS*PPPPNPVVK −0.90 0.001 −0.11 0.599
Q8CHT1 Ngef 84 Ephexin-1 RAS*DQADLPK −0.88 0.002 0.32 0.179
Q8CI52 Gramd1c 531 Protein aster-C S*STDLGFEAK −0.87 5.31 × 10−5 0.08 0.558
P43277 Hist1h1c;
Hist1h1d		 37 Histone H1.2; histone H1.3 KAS*GPPVSELITK −0.84 0.003 0.26 0.263
Q8C341 Suco 1069 SUN domain-containing ossification factor RTS*FPLIR −0.80 0.006 0.13 0.597
Q8K4L3 Svil 857 Supervillin KLS*VDNNTSATDYK −0.77 0.009 −0.03 0.918
Q67FY2 Bcl9l 118 B cell CLL/lymphoma 9-like protein SVS*VDSGEQR −0.75 0.002 −0.86 0.001
F6ZDS4 Tpr 2204 Nucleoprotein TPR TVPS*TPTLVVPHR −0.74 0.004 −0.47 0.047
Q8C079 Strip1 59 Striatin-interacting protein 1 KDS*EGYSESPDLEFEYADTDK −0.73 0.001 0.08 0.642
Q9JKB3 Ybx3 328 Y-box-binding protein 3 S*RPLNAVSQDGK −0.72 0.004 0.09 0.644
Q6NZF1 Zc3h11a 740 Zinc finger CCCH domain-containing protein 11A RLS*SASTGKPPLSVEDDFEK −0.71 0.006 −1.00 0.001
O35609 Scamp3 78 Secretory carrier-associated membrane protein 3 KLS*PTEPR −0.71 0.004 0.18 0.389
P15066 Jund; Jun 100 Transcription factor jun-D LAS*PELER −0.69 0.002 0.07 0.720
Q02248 Ctnnb1 552 Catenin β1 RTS*M^GGTQQQFVEGVR −0.69 0.003 −0.15 0.455
Q8BUH8 Senp7 12 Sentrin-specific protease 7 RAS*SEIVTEGK −0.67 8.08 × 10−5 −0.67 8.49 × 10−5
Q6PGG2 Gmip 260 GEM-interacting protein S*REEAQAK −0.67 0.001 0.33 0.057
Q04899 Cdk18 75 Cyclin-dependent kinase 18 RLS*LPMDIR −0.66 0.005 −0.40 0.057
P20357 Map2 1635 Microtubule-associated protein 2 S*GILVPSEK −0.65 0.005 −0.66 0.004
Q61165 Slc9a1 707 Sodium/hydrogen exchanger 1 IGS*DPLAYEPK −0.63 0.004 0.17 0.366
Q8K3X4 Irf2bpl; Irf2bp2 13 Probable E3 ubiquitin-protein ligase IRF2BPL; interferon regulatory factor 2-binding protein 2 RQS*CYLCDLPR −0.63 0.001 0.33 0.047
P70698 Ctps1 575 CTP synthase 1 SGSSS*PDSEITELK −0.61 0.009 0.36 0.089
A0A087WQ44 Srcap 2771 Snf2-related CREBBP activator protein TS*ADVEIR −0.59 0.004 −0.49 0.011
P81122 Irs2 362 Insulin receptor substrate 2 TAS*EGDGGAAGGAGTAGGR −0.58 0.004 0.19 0.249
Q02248 Ctnnb1 551 Catenin β1 RT*SM^GGTQQQFVEGVR −0.57 0.008 −0.06 0.754
F7BJB9 Morc3 563 MORC family CW-type zinc finger protein 3 RLS*NPPVENSSYK −0.57 0.002 −0.29 0.062
Q811L6 Mast4; Mast1; Mast2 1436 Microtubule-associated serine/threonine-protein kinase 4; microtubule-associated serine/threonine-protein kinase 2 SAEPPRS*PLLK −0.56 0.001 −0.41 0.007
Q8BG05 Hnrnpa3 361 Heterogeneous nuclear ribonucleoprotein A3 SSGSPY*GGGYGSGGGSGGYGSR −0.54 0.003 −0.23 0.143
P35569 Irs1 3 Insulin receptor substrate 1 AS*PPDTDGFSDVR −0.54 2.04 × 10−4 −0.09 0.418
P97310 Mcm2 21 DNA replication licensing factor MCM2 RIS*DPLTSSPGR −0.51 0.001 −0.11 0.378
Q9QXZ0 Macf1 3889 Microtubule-actin cross-linking factor 1 QGS*FSEDVISHK −0.51 0.008 0.23 0.177
Q8K310 Matr3 188 Matrin-3 RDS*FDDR −0.50 0.003 0.11 0.423
Q9DBR1 Xrn2 473 5′-3′ exoribonuclease 2 NSSPS*ISPNTSFASDGSPSPLGGIK 0.50 0.003 −0.41 0.010
E1U8D0 Soga1 1300 Protein SOGA1 APS*PTTAAGEESCK 0.52 0.006 0.11 0.494
Q8BTI8 Srrm2 1338 Serine/arginine repetitive matrix protein 2 S*SSELSPEVVEK 0.53 0.006 0.10 0.561
Q8BIA4 Fbxw8 86 F-box/WD repeat-containing protein 8 SRS*PPDRDATEPEPLVDQLIR 0.57 0.003 −0.24 0.126
Q99K30 Eps8l2 217 Epidermal growth factor receptor kinase substrate 8-like protein 2 QPGDS*PQAK 0.57 0.007 −0.02 0.893
P63058 Thra 12 Thyroid hormone receptor α VECGS*DPEENSAR 0.61 0.006 −0.24 0.208
Q9CQT2 Rbm7 108 RNA-binding protein 7 SGSSHASQDASVSYPQHHVGNLS*PTSTSPNSYER 0.63 0.008 −0.10 0.608
Q9WTQ5 Akap12 584 A-kinase anchor protein 12 GPSEAPQEAEAEEGATS*DGEKKR 0.64 0.008 −0.02 0.908
Q9QXM1 Jmy 704 Junction-mediating and -regulatory protein STAS*PVPCEEQCHSLPTVLQGQEK 0.67 0.005 −0.29 0.166
P39053 Dnm1 774; 777 Dynamin-1 RS*PTS*SPTPQR 0.67 0.002 −0.31 0.105
Q9Z1T6 Pikfyve 1753 1-Phosphatidylinositol 3-phosphate 5-kinase GTAGKS*PDLSSQK 0.68 5.23 × 10−5 −0.25 0.039
Q8BTI8 Srrm2 1343 Serine/arginine repetitive matrix protein 2 SSSELS*PEVVEK 0.68 0.002 0.13 0.472
Q8C078 Camkk2 91 Calcium/calmodulin-dependent protein kinase kinase 2 DQPPEADGQELPLEASDPESRS*PLSGR 0.75 9.91 × 10−5 −0.14 0.317
P58802 Tbc1d10a 407 TBC1 domain family member 10A AILDAEPGPRPALQPS*PSIR 0.78 2.06 × 10−4 −0.34 0.039
P43274 Hist1h1c;
Hist1h1t;
Hist1h1e;
Hist1h1b;
Hist1h1d		 102 Histone H1.2;
histone H1.4;
histone H1.5;
histone H1.3		 GTGAS*GSFK 0.79 0.006 0.10 0.663
Q9ESE1 Lrba 979 Lipopolysaccharide-responsive and beige-like anchor protein DS*PISPHFTR 0.80 0.003 −0.10 0.659
Q6PDN3 Mylk 355 Myosin light chain kinase, smooth muscle VPAIGSFS*PGEDRK 0.81 0.001 −0.21 0.261
P56402 Aqp2 256; 261 Aquaporin-2 QS*VELHS*PQSLPR 1.02 4.83 × 10−4 0.44 0.064
P40645 Sox6 454 Transcription factor SOX-6 TS*PVNLPNK 1.19 0.007 −0.10 0.783
P40645 Sox6 439 Transcription factor SOX-6 S*PTSPTQNLFPASK 1.40 0.002 0.13 0.708

PKA-Cβ-null data for the same peptides are given for comparison. Pmod value, moderated P value.

†

Multiple gene symbols are given when a peptide matches sequences in multiple proteins. *Phosphorylated amino acids.