Fig. 1.

Clear, Unobstructed Brain/Body Imaging Cocktails and Computational (CUBIC)-clarified mouse kidneys. A: paraformaldehyde-fixed kidneys were cleared using CUBIC solutions (2-mm grid spacing). B: a representative image of 300-μm-thick sagittal kidney sections from male and female mice labeled with antibodies to parvalbumin (green) and aquaporin 2 (red). Scale bar = 1 mm. C: kidney circumference in male and female mice. D: CUBIC expanded the kidney circumference to the same extent in both sexes. Data are presented here as means ± SE; n = 5–6 animals/group. A Student’s t test was performed, and *P < 0.05 was considered statistically significant.