Fig. 4.

Physiological responses to furosemide treatment. A−D: furosemide- induced diuresis (A) and urinary excretion of Na⁺ (B), Cl⁻ (C), and K⁺ (D) in male and female mice. E−H: plasma electrolyte panel postfurosemide treatment [plasma Na⁺ (E), Cl⁻ (F), K⁺ (G) and ${\text{HCO}}_3^{-}$ (H)]. P < 0.05 was considered statistically significant *between sexes and #between treatments, respectively. Data are presented as means ± SE; n = 6 animals/group.