Fig. 7.

Remodeling of distal convoluted tubule (DCT) volume. A: surface rendering of parvalbumin- expressing DCTs highlighting the variations in size and shape of the DCT. B and C: violin plots showing the distribution of individual tubule measurements and medians (dashed lines) and upper and lower quartiles (dotted lines) of the tubule volume (B) and tubule cross-sectional area (C) of vehicle- and furosemide-treated male mice (blue) and female mice (pink). Four kidneys per group and 15 DCTs per kidney were analyzed. Two-way ANOVA tests were performed to test for interactions, with Tukey post hoc analysis, and P < 0.05 was considered significant (*between sexes in the basal state; #effect of furosemide in the same sex, and **between sexes in the furosemide-treated state, respectively).