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. 2020 Sep 21;319(5):F773–F779. doi: 10.1152/ajprenal.00427.2020

Fig. 2.

Fig. 2.

Formoterol (form) improves mitochondrial function and dynamics proteins in glucose-treated renal proximal tubule cells (RPTCs). RPTCs were grown in 0 mM glucose, 17 mM mannitol, or 17 mM glucose and cotreated with formoterol (30 nM) for 96 h. The Seahorse XF96e Analyzer was used to measure FCCP-oxygen consumption rate (OCR; A), electron transport chain (ETC) complex III [antimycin A (AA); B], and complex I (rotenone) function (C). D−G: immunoblot (D) and densitometry analysis of phosphorylated (p) dynamin-related protein 1 (Drp1) S637 to total Drp1 (E), mitofusin 1 (Mfn1) expression (F), and mitofusin 2 (Mfn2) expression (G). Data are presented as means ± SE; n = 6–8. Statistical significance was determined by two-way ANOVA and Tukey-Kramer post hoc test. αP < 0.05 compared with 0 mM glucose vehicle (Veh); βP < 0.05 compared with 17 mM glucose vehicle.