Fig. 3.

Localization of Tregs (Foxp3-positive cells) (brown cells, arrows) in the brain and spleen of experimental autoimmune encephalomyelitis (EAE) mice. Staining was developed using diaminobenzidine substrate and sections were counterstained with hematoxylin and eosin. Representative brain sections are shown from: healthy control (A), initial attack (B), and remission (C) of the disease; section from the spleen (D) served as a positive control for staining (original magnification 20×)