Figure 4.

Receiver-operating characteristic curve results of responses from TRXL1–GST multiplex bead assays using different detection reagents. Curves were constructed as described in Figure 3. The optimal threshold for the TRXL1–GST multiplex assay with the rabbit anti-IgG Fc polyclonal antibody was 2,283 MFI-bg with a sensitivity of 74.4% and a specificity of 75.6%. The optimal threshold with the goat anti-IgG₁ polyclonal antibody was 17,465 MFI-bg with a sensitivity of 61.5% and a specificity of 67.5%. The optimal threshold with the anti-IgG₂ monoclonal antibody was 775 MFI-bg with a sensitivity of 64.1% and a specificity of 61.0%. The optimal assay threshold for the with the 4E3D9 monoclonal antibody was 161.5 MFI-bg with a sensitivity of 74.4% and a specificity of 80.5%. MFI-bg = median fluorescent intensity minus background; TRXL1 = thioredoxin-like protein 1. This figure appears in color at www.ajtmh.org.