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. 2020 Dec;63(6):767–779. doi: 10.1165/rcmb.2019-0085OC

Figure 1.

Figure 1.

Effects of cigarillo (CLLO) smoke on human tracheobronchial epithelial (HTBE) cell viability and layer integrity. (A) Representative images of propidium iodide (red) uptake by chronic smoke–exposed HTBE cells showing calcein acetoxymethyl (AM) staining (green) for live cells after CLLO-smoke exposure. (B) Quantitation of the number of propidium iodide–positive cells per image (n = 3 donors, 5–10 random images/culture were captured and quantitated). (C) Transepithelial electrical resistance (n = 6 donors, approximately six individual culture inserts per donor code were measured) after CLLO-product (Swisher Sweets CLLO [SSW], Garcia y Vega Game black CLLO [GBK], and Hi-Fi Tropical Tango CLLO [HTT]) and Kentucky research cigarette (KCS) smoke exposure. Data are expressed as the mean + SEM. *P ≤ 0.05, ***P ≤ 0.005, and ****P ≤ 0.001 by one-way ANOVA. TEER = transepithelial electrical resistance.