Figure 6.

Lungs from both female and male mouse offspring (6-wk old) from mothers prenatally exposed to e-cig aerosols with or without nicotine were homogenized, and lipogenic/myogenic markers were analyzed by quantitative real-time-PCR. The mRNA expression levels of PPARg (peroxisome proliferator–activated receptor γ), ADRP (adipose differentiation related protein), CEBPA (CCAAT enhancer–binding protein α), CNN1 (calponin 1), ACTA2 (α-smooth muscle actin), FN1 (fibronectin), SERPINE1 (plasminogen activator inhibitor-1), and MMP9 were measured in both (A) female and (B) male pup lungs. β-tubulin/GAPDH was used as an endogenous control for normalization (n = 4–6 for both female and male mice). *P < 0.05 and **P < 0.01.