Figure 2.

scRNA-seq analysis identifies discrete renal cell types, with dynamic changes in the proportion and transcriptome of each cell type observed across the R-UUO model. tSNE plots of 17,136 cells from libraries pooled from mice that underwent sham, UUO-2, UUO-7, or R-UUO (2 weeks) (n=3 per time point) classified by (A) cell cluster and (B) time point. (C) Expression of selected marker genes for each cell classification projected onto tSNE plot. Color scale is log₁₀ expression levels of genes. (D) Relative proportions of cells assigned to each cluster by time point. Statistical significance derived using differential proportional analysis, with a mean error of 0.1 over 100,000 iterations. *P<0.05. (E) Violin plots of Egf and Lcn2 (encodes neutrophil gelatinase-associated lipocalin) gene expression in the loop of Henle/distal convoluted cell cluster. The y axis shows the log-scale normalized read count. a–c, PCT subclusters colored by shared nearest neighbor; DCT, distal convoluted tubule; LoH, loop of Henle; Mac, macrophage; Mono, monocyte; NK, natural killer cell; PCT, proximal convoluted tubule; S1, S1 segment.