Figure 5.

Mannose receptor (MR)⁺ F4/80^Hi macrophages and MMP12⁺F4/80^Lo cells are observed in late injury or specifically following reversal of obstruction, respectively. (A) Representative immunofluorescence images across the R-UUO time course (sham, UUO-2, UUO-7, and R-UUO [2 weeks]) for mannose receptor (MR, marker of Mrc1⁺ macrophages, green) and F4/80 (red). (B) Single R analysis comparing the transcriptome of the myeloid clusters with those of embryonic macrophages, adult macrophages before and after renal IRI, and infiltrating monocytes during repair of renal IRI. (C) Immunofluorescence images showing colocalisation of MMP12 (green) and F4/80 (red) in renal macrophages. (D) Flow cytometry plots of bone marrow–derived macrophages (BMDMs) demonstrating fluorescence after phagocytosis of FITC-collagen. (E) Expression of reparative macrophage genes measured by quantitative RT-PCR in BMDMs after phagocytosis of FITC-collagen versus BMDMs cultured in medium alone (control). n=4 replicates. *P<0.05, **P<0.01. Mac, macrophage.