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. 2021 Jan 7;16(1):e0223288. doi: 10.1371/journal.pone.0223288

Fig 3. CRISPR/Cas9-induced cell death in HPV 16-driven xenografts is not immunogenic.

Fig 3

(A) Examination of H&E stained tumour specimens from Cas9+16E7 treated mice (16E7 #1 group) for inflammatory cells infiltration. (B) Extracellular ATP release assay of TC1 cells (HPV 16+ve) treated with Cas9+16E7, Cas9+nonspecific gRNA, or untreated, over 72 hours. A total of 800 ng of plasmid DNA was transfected per well (24-well plate) at 70% cell confluency. (C) TC1 cells were treated with Cas9+16E7, Cas9+nonspecific gRNA (control), or untreated for 72 hours before protein release of HMGB-1 was assessed by western blot analysis. Samples were collected from adherent cells (adh) or supernatant (snt) for each group. HeLa cells lysate was used as positive control. Data were represented as mean ± SD. Statistical significance was assessed by ANOVA with post-hoc analysis. * p<0.05, ** p<0.01, *** p<0.001.