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. 2021 Jan 7;17(1):e1009033. doi: 10.1371/journal.ppat.1009033

Fig 7. KSHV orf10 interacts with p53 in KSHV infected cells.

Fig 7

(A) A293T.219 cells were transfected with pDEST47-KSHV orf10-Flag. At 16h p.t., the cells were induced with 1mM NaB and 25 ng/ml 12-O-tetradecanoylphorbol-13-acetate (TPA) for 48h. Stimulation of A293T.219 cells was monitored by RFP expression. Transfected stimulated and unstimulated cells were imaged with bright field and fluorescence microscopy to visualize upregulation of expression of RFP and cytopathic effect (CPE) induced by KSHV replication. Panel A—C show mock-transfected, uninduced cells, panels D—I depict KSHV ORF10-FLAG transfected cells that were either left untreated or induced. Panels (J) KSHV orf10 coimmunoprecipitates with p53. p53 was immunopreciptated from whole cell lysates with antibody binding to p53 C-terminus. Presence of orf10-Flag and p53 in the lysates and co-imunoprecipitated fractions was tested with mouse anti-Flag and mouse anti-p53 DO7 Ab.