Fig 1. Extracellular concentration of toxin A (TcdA) excreted by C. difficile in CAM supplemented with different sugar derivatives, sugar alcohols and L-lactate.
Shown are concentrations of TcdA in ng/mgCDW in the supernatant of C. difficile 630Δerm grown in casamino acids medium (CAM, reference) and CAM supplemented with different sugar derivatives, sugar alcohols and L-lactate. The TcdA concentrations were determined by ELISA after 48 h of cultivation. All TcdA concentrations measured after cultivations in media with different additives were significantly altered compared to that determined in the control medium CAM without any additions (p-value < 0.01).