Fig 5. Scatterplots of the intracellular and extracellular metabolome analysis of C. difficile grown in absence and presence of L-lactate.

Shown are the detected intracellular metabolites of the exponential (A) and the stationary phase (B) samples as well as the detected extracellular metabolites of the exponential (C) and the stationary phase (D) samples of C. difficile 630Δerm cultivated in casamino acids medium without (CAM) or supplemented with 500 mg/L L-lactate (CAM+L). The majority of metabolite concentrations remained unchanged (p-value < 0.05) for the two tested conditions. Metabolites detected only in one condition were shown in boxes. All experiments are based on four independent cultivations.