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. 2021 Jan 7;16(1):e0245175. doi: 10.1371/journal.pone.0245175

Fig 2. Agarose gel electrophoretic analysis of amplified DNA fragments generated from multiplex and singleplex PCR assays.

Fig 2

(A) Multiplex PCR amplification of DNA templates from B. thailandensis strains E264, DV1 (lanes 1–2), the BTCV strains E555, SBXPR001 (lanes 3–4), BPBM strains MSHR3326, MSHR4445 (lanes 5–6), B. pseudomallei strains K92643, 1026b (lanes 7–8), B. mallei strains NCTC3709, NCTC12938 (lanes 9–10), and B. humptydooensis strains MSMB43, MSMB1588 (lanes 11–12). (B) Singleplex PCR amplification of B. thailandensis strains E264, DV1 (Bth; lanes 1–2) and B. thailandensis strain E555, SBXPR001 (BTCV; lanes 3–4). Lanes P and N are positive (mixture of Burkholderia spp. DNA) and negative (distilled water) controls, respectively. Lane M is 100 bp DNA ladder.