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Various concentrations (1, 10, and 100 ng/μl) of each Burkholderia genomic DNA were subjected to multiplex PCR including B. pseudomallei K92643 (Bps; 963 bp), B. pseudomallei MSHR491 (BPBM; 586 bp), and B. thailandensis E264 (Bth; 139 bp). Lanes M and N represent 100 bp DNA ladder and negative control (distilled water), respectively.
