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. 2021 Jan 7;16(1):e0245175. doi: 10.1371/journal.pone.0245175

Fig 4. Multiplex PCR assay to detect B. pseudomallei, BPBM and B. thailandensis in spiked soil samples.

Fig 4

Ten-fold serial dilution (lanes 1–3) of B. pseudomallei, BPBM and B. thailandensis were spiked into 20 g soil sample before DNA extraction and multiplex PCR. Lanes P (mixture of Burkholderia spp. genomic DNA) and N (no added bacteria) are positive and negative controls, respectively. Lane M is 100 bp DNA ladder.