Table 2. Oligonucleotide primers and the size of bimA amplicons for detection of B. pseudomallei, B. thailandensis, B. mallei and their variants including BTCV and BPBM.
| Primer name | Sequences 5′ to 3′ | Size of amplicon | For detection of | References |
|---|---|---|---|---|
| BimABps-F | GATCGCTGAAGAAAAATCCG | 963 bp | B. pseudomallei | This study |
| BimAcom-R | CCTTGAGGTTTTCGTTGATG | |||
| BimABPBM-F | ATTCCTAACGCGACACCAAC | 586 bp | BPBM and B. mallei | This study |
| BimAcom-R | CCTTGAGGTTTTCGTTGATG | |||
| BimABth-F | ATCCGAACGAAACACGCG | 139 bp | B. thailandensis and BTCV | This study |
| BimABth-R | TTCGTCGTCCGACCATGA | |||
| BimABth-SF | AGGCGGGTAATCGACTCA | 87 bp | B. thailandensis | This study and [48] |
| BimABth-R | TTCGTCGTCCGACCATGA | |||
| 27F | AGAGTTTGATCMTGGCTCAG | 527 bp | E. coli | [45] |
| 518R | ATTACCGCGGCTGCTGG | 16S rRNA |