Table 3. Specificity of the multiplex PCR assay against 121 isolates of Burkholderia spp. and 24 isolates of Gram-positive and Gram-negative bacteria.
| Bacteria | Source [References] | No. of isolates | No. of PCR positive/ Total No. of isolates (%) |
|---|---|---|---|
| Burkholderia spp. | |||
| B. pseudomallei | Clinical [29, 30, 32, 49–52] | 18 | 9/9 (100%) |
| Environment [11] | 17 | 24/24 (100%) | |
| BPBMa | Clinical [20, 33] | 20 | 20/20 (100%) |
| B. thailandensis | Environment [10, 11, 34] | 30 | 30/30 (100%) |
| BTCVb | Environment [11] | 10 | 10/10 (100%) |
| B. mallei | Animal [53] | 2 | 2/2 (100%) |
| B. humptydooensis | Environment [3, 36] | 2 | 2/2 (100%) |
| B. multivorans | Environment [38, 54] | 3 | 0/3 (0%) |
| B. ubonensis | Environment [36, 39, 55] | 3 | 0/3 (0%) |
| B. anthina | Environment [36] | 2 | 0/2 (0%) |
| B. cepacia | Clinical [36, 37] | 2 | 0/2 (0%) |
| Environment [36, 56] | 5 | 0/5 (0%) | |
| B. cenocepacia | Environment [36] | 2 | 0/2 (0%) |
| B. diffusa | Environment [36] | 2 | 0/2 (0%) |
| B. territorii | Environment [36] | 2 | 0/2 (0%) |
| B. pseudomultivorans | Environment [36] | 1 | 0/1 (0%) |
| B. oklahomemsis | Clinical [40] | 1 | 0/1 (0%) |
| B. vietnamiensis | Clinical [38, 55] | 1 | 0/1 (0%) |
| Non-Burkholderia spp. | |||
| S. aureus | Clinical | 1 | 0/1 (0%) |
| Food | 4 | 0/4 (0%) | |
| P. aeruginosa | Environment | 10 | 0/10 (0%) |
| E. coli | Environment | 5 | 0/5 (0%) |
| A. baumannii | Environment | 4 | 0/4 (0%) |
aB. pseudomallei that expresses a B. mallei-like bimA; bB. thailandensis that expresses a B. pseudomallei-like capsule.