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. 2021 Jan 7;12(1):53. doi: 10.1038/s41419-020-03283-2

Fig. 3. IL-38 expression in the lung is upregulated upon poly(I:C) exposure in vivo and ameliorates poly(I:C)-induced acute lung injury.

Fig. 3

C57BL/6 mice (n = 5) were injected intranasally with poly(I:C) (10 mg/kg/mouse) with/without recombinant murine (rm) IL-38 (800 ng/injection) per day. (a) IL-38 mRNA levels in the lung were measured by quantitative real-time PCR (QT-PCR). (b) IL-38 protein levels in the lung were detected by immunohistochemistry. (c) Total protein concentrations in the BALF were measured with BCA protein assay kit. (d) Representative result of hematoxylin and eosin (H&E)-stained lung from mice (n = 5 per group) treated with or without rmIL-38 at 24 h, 4 days and 7 days after poly(I:C) injection. (e) Histological scores for lung injury assessment in the mice treated with or without rmIL-38 at 24 h, 4 days, and 7 days after poly(I:C) injection. Data are shown as the mean ± SEM of three duplicate tests. Nonparametric Kruskal-Wallis test followed by Dunn’s multiple comparisons test and/or Mann-Whitney test was used to compare the differences between groups. *P < 0.05, **P < 0.01, and ***P < 0.001.