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. 2021 Jan 7;12(1):53. doi: 10.1038/s41419-020-03283-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Bronchoalveolar lavage fluid (BALF), lung, and spleen (n = 5 each group) were collected on day 1, 4, and 7 after poly(I:C) injection. (a) Gating strategies for the analysis of myeloid cells from BALF cells based on CD11b, F4/80, and Ly6G expressions. (b) Number of total cells, neutrophils, and macrophages were counted at indicated times. (c, k) Gating strategies for the analysis of lymphoid cells from lung and spleen, respectively. Cells were gated based on CD45, CD3, CD4, CD8, γδ-TCR and NK1.1, IFN-γ, IL-4, IL-17A, CD25, and Foxp3 expression for lymphoid populations. DRAQ7 was used to exclude dead cells. (d–j, l–q) Percentage of lymphoid cell populations in lung and spleen at indicated time points. Each symbol represents an individual mouse. Data (means ± SEM) are representative of three independent experiments. Mann-Whitney test was used to compare the differences between groups. *p < 0.05, **p < 0.01, and ***p < 0.001.